0000087: frame averaging with Flash4 crashes Pol-Acquisition plugin

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Date Submitted

Last Update

0000087

OpenPolScope-API

[All Projects] bug

public

2019-01-07 12:12

Reporter

rudolfo

Assigned To

amit

Priority

high

Severity

major

Reproducibility

always

Status

assigned

Resolution

open

Platform

Nikon inverted, Ham Flash4

Windows

OS Version

Product Version

Target Version

Fixed in Version

Summary

0000087: frame averaging with Flash4 crashes Pol-Acquisition plugin

Description

On Friday, Jan 4, Mai and I used the Nikon inverted stand with the light field camera that now has the new Hamamatsu Flash4 camera with USB connection installed. The Pol-Acquisition plugin in polarized fluorescence mode worked without a hitch, except when we enabled frame averaging. With frame averaging enabled (4 frames), a time series acquisition stalled halfway, and MicroManager needed to be rebooted. Frame averaging seemed to work for some of the acquisitions, but then failed.

Following this experience, we disabled frame averaging (we only recorded sample images, no background was needed), and the rest of the session, taking about 6 hours with many (beautiful) data sets, went flawlessly.

Issue History
Date Modified	Username	Field	Change
2019-01-07 12:12	rudolfo	New Issue
2019-01-07 12:12	rudolfo	Status	new => assigned
2019-01-07 12:12	rudolfo	Assigned To	=> amit

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